Computational Analysis of Heat shock Protein 27 (HSP27) from different source organisms
DOI:
https://doi.org/10.21015/vtse.v10i1.859Abstract
Heat shock protein 27 (HSP27) also called HSPB1 is a member of small heat shock protein (sHsps). HSB1 helps in developing a stable state during stress conditions like heat shock or oxidative injuries. Several studies have performed for the identification of HSPB1 function. However, limitation to highlight structural and phylogenetic relationships of HSPB1 in various organisms. Therefore, the aim of this study to investigate HSPB1 protein in twelve different organisms namely Homo Sapiens (Human), Dugesia japonica (Flatworms), Sus scrofa (Pig), Carassius auratus (Goldfish), Oreochromis niloticus (Nile Tilapia), Rattus norvegicus (Norway Rat), Xenopus laevis (African clawed Frog), Canis lupus familiaris (Dog), Musca domestica (House Fly), Phenacoccus solenopsis (Solenopsis mealybug), Kryptolebias marmoratus (mangrove rivulus\fish) and Alligator mississippiensis (American alligator) by identifying and analyzing the Multiple sequence alignment (MSA), similarity matrix, physiochemical properties, phylogenetic relationship, secondary and tertiary structure, predict motifs and domains, analyze gene structure through several tools..For this purpose organisms were predicted based on soluble and hydrophilic in nature.Results showed all organisms had upstream, downstream and CDS part in their protein sequences except Kryptolebias marmoralus (fish), Oreochromis Niloticus (Nile Tilapia),Phenacoccus Solenopsis (Solenopsis mealybug), and Sus Scrofa (Pig) which had only CDS part. The main domain that was found in all organisms except Phenacoccus were A-Crystalline(IPR002068) and the homologous super family in all organisms were Hsp20 (IPR002068).In phylogenetic tree two clades formed and in the endidentified that Rattus norvegicus and Canis lupus are more similar with each other as they share many common features. Moreover Homo Sapiens, Sus scrofa and Canis lupus, Sus scrofa were found to be more similar. By performing different analysis it was predicted that Phenacoccus Solenopsis was not related to any organism in many aspects. However,limitation to predict quaternary structures of organisms. The Results shows HSPB1 gene has identical homologue, functional similarity and highly conserved among these organisms.
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