Comparative Computational Analysis of a Putative Transcriptional Regulator Map_PRSO3010 and its implications in the Pathogenesis of Crohn’s and Johne’s diseases

Syed Asif Hassan


The implications of specific biochemical and structural features of many putative virulence factors in the pathogenesis of MAP in ruminant and human being are relatively less explored and understood. In this background, the comparative genomic and proteomic studies of MAP_PRSO3010, a putative protein shows that it shares a sequential, physiochemical, structural and functional similarity with Rv0757 (PhoP) protein, a virulent molecular determinant of Mycobacterium tuberculosis (Mtb). Conserved Domain Database (CDD) and InterPro studies demonstrate the presence of conserved domains namely Signal transduction response regulator, receiver domain (REC) and Transcription regulatory protein, C-terminal (Trans_reg_C) belonging to CheY-like and Helix Turn Helix (HTH) superfamily, respectively in both MAP_PRSO3010  and Rv0757 proteins. These domains are mainly involved in phosphorelay signal transduction and transcriptional regulation in response to unfavorable environments within host macrophages. Comparative in silico protein-protein interaction (PPI) studies also showed the involvement of common interacting proteins namely mtrA/B crucial for the survival of Mtb within host macrophages. The predicted hypothetical model of MAP_PRSO3010 protein provides an insight on the functional and structural resemblance between the two proteins. The model quality and structure assessment tools of Swiss-Model Server also validated the predicted hypothetical structure of MAP_PRSO3010 protein. Thus, these results show a strong relevance of MAP_PRSO3010 protein in MAP virulence

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